Fig. 2.

Interrogation of LMS function, structure and biochemistry. Several assays can be used to interrogate the function, structure and biochemistry of myocardial slices. Functional assessment includes contractile function, conduction velocity, Ca²⁺ handling, action potential, metabolomics and viability. Structural assessment can be conducted using traditional sectioning and staining methods. Additionally, immunohistochemical staining and microscopy can easily be performed and optical clearing protocols have been developed for whole slice imaging. Biochemistry assays can be conducted at the genomic, proteomic and secretomic levels. Longitudinal functional assessment of LMS can also be performed during chronic culture. The RNA sequencing image in this figure was previously published [43]