Figure 2.

Expression of transgelin and signalling proteins upon treatment of podocytes with high glucose. Protein levels of transgelin, cleaved caspase 3, Smad3 and phospho‐Smad3 protein were assessed by Western blot. β‐Actin was used as an internal control. A, Representative blot, B, Average levels of transgelin, C, cleaved caspase 3, D, phospho‐smad3 and E, total smad3 were normalized with respect to β‐Actin. Data are representative of three independent experiments, and were analysed using one‐way analysis of variance. *P < .05, **P < .01 compared with control or mannitol group