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Generation of aAPCs. The aAPCs generated with human DCs and PLGA-NPs encapsulated SV95 peptide and coumarin 6 were imaged under a scanning electron microscope (SEM). (A) SEM image of the PLGA-NPs with a magnification of 11,112, scale bar = 200 nm. The aAPCs were analyzed using a laser scanning confocal microscope (Leica TCS SP2, Buffalo Grove, IL, United States). (B) DAPI channel; (C) FITC channels; (D) DAPI and FITC channels were overlaid. Scale bar = 10 μm.
