FIGURE 5.

Cytotoxicity assay of the SV₉₅-CTL lines. Cytotoxicity assay was performed to test SV₉₅ specific CTL lines. SV₉₅ peptide pulsed T2 cells were used as the target cells. In addition, both LNCap (HLA-A2 positive cell line) and PC-3 (HLA-A2 negative cell line) were also used as the target cells in this cytotoxicity assay. (A) SV_95–1 peptide pulsed T2 cells were significantly eliminated by both SV_95–6 and SV_95–7 specific CTL lines (p < 0.05, p < 0.01, two-tailed, student’s t test). (B) Prostate cancer LNCap cells were significantly depleted by both SV_95–6 and SV_95–7 specific CTLs (p < 0.01, two-tailed, student’s t test). (C) Lung cancer A549 cells, breast cancer MCF-7 cells were significantly eliminated by SV_95–6 specific CTL line (p < 0.01, two-tailed, student’s t test). In addition, activated HLA-A2 (+) PBMCs could also be killed by SV_95–6 specific CTL line (p < 0.05, two tailed, student’s t test), but HLA-A2 (+) stem cells were not significantly killed. All the statistics were conducted with one-way ANOVA. *p < 0.05, **p < 0.01.