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. 2020 Sep 2;7:570003. doi: 10.3389/fmolb.2020.570003

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Copyright © 2020 Chen, Jia, Zhao, Bao, Zhang, Ozkan, Minev and Ma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MHC Class I restriction assay. MHC class I restriction assay was performed by a cytotoxic assay of testing SV₉₅-specific CTL lines against SV_95–1 peptide-pulsed T2 cells with the addition of blocking antibody against MHC class I molecule (anti-HLA-A2, BB7.2), or MHC class II molecule (anti-MHC class II, IVA 12). SV_95–6 specific CTL clone was a representative among the SV₉₅ CTL lines. The cytotoxicity of SV_95–6 specific CTLs was completely blocked by MHC I antibody, but not at all by MHC II antibody.