Figure 6.

SUMO2/3 conjugation to HCN2 channels is increased in small diameter neurons from ipsilateral relative to contralateral DRG at 1 day post‐CFA. (a) Representative PLA. Scale bars are 25 µm. Note that this image provides an overview, but for the purpose of quantification, individual projections were made for each cell, for example, Figure 5b. (b) Plots of mean number of puncta/µm² for all DRG from six CFA treated animals on day 1 post‐CFA. The number inside the bar represents the total number of cells analysed. Inset: Each line compares the means for contralateral and ipsilateral DRG from one animal. Paired t‐tests indicate the number of puncta significantly increased for small but not medium or large neurons in ipsilateral compared to contralateral DRG (small, 0.23 ± 0.02 vs. 0.29 ± 0.04, p = 0.0499; medium, 0.24 ± 0.02 vs. 0.26 ± 0.02, p = 0.2120; large, 0.26 ± 0.03 vs. 0.31 ± 0.04, p = 0.4259), ^* p < 0.05. (c) Plots of mean puncta intensity on day 1 post‐CFA. Paired t‐tests indicate mean puncta intensities were not significantly different between ipsilateral and contralateral DRG for any size category (small, 44.47 ± 3.1 vs. 41.86 ± 3.9, p = 0.4495; medium, 43.06 ± 2.5 vs. 42.43 ± 4.1, p = 0.8503; large, 43.74 ± 3.1 vs. 44.51 ± 4.8, p = 0.8518). CFA, Complete Freund's Adjuvant; DRG, dorsal root ganglia; HCN2, hyperpolarization‐activated, cyclic nucleotide‐gated 2; PLA, proximity ligation assays; SUMO, small ubiquitin like modifier