Figure 8.

HCN2 channel SUMOylation by SUMO1 is diminished in medium and large diameter neurons from ipsilateral relative to contralateral DRG at 1 day post‐CFA. (a) Representative PLA. Scale bars are 25 µm. Note that this image provides an overview, but for the purpose of quantification, individual projections were made for each cell, for example, Figure 5b. (b) Plots of mean number of puncta/µm² for all DRG from six CFA treated animals on day 1 post‐CFA The number inside the bar represents the total number of cells analysed. Inset: Each line compares means for contralateral and ipsilateral DRG from one animal. Paired t‐tests indicate medium and large diameter cells contain significantly fewer puncta in the ipsilateral DRG relative to the contralateral DRG (small, 0.33 ± 0.05 vs. 0.27 ± 0.03, p = 0.2058; medium, 0.34 ± 0.05 vs. 0.24 ± 0.03, p = 0.0480; large, 0.37 ± 0.05 vs. 0.26 ± 0.02, p = 0.0238), ^* p < 0.05. (c) Plots of mean puncta intensity on day 1 post‐CFA. Paired t‐tests indicate mean puncta intensities were not significantly different for any size category in ipsilateral compared to contralateral DRG (small, 44.68 ± 4.03 vs. 41.48 ± 2.66, p = 0.5986; medium, 42.7 ± 3.24 vs. 45.65 ± 4.06, p = 0.6299; large, 44.44 ± 3.68 vs. 46.39 ± 4.27, p = 0.7631). CFA, Complete Freund's Adjuvant; DRG, dorsal root ganglia; HCN2, hyperpolarization‐activated, cyclic nucleotide‐gated 2; PLA, proximity ligation assays; SUMO, small ubiquitin like modifier