FIGURE 4.

Endoglycosidase of Streptococcus pyogenes (EndoS)‐imlifidase allows hematopoietic chimerism in presensitized recipients. A, Schematic of the chimerism induction protocol; naive B6.H‐2^g7 or NOD mice were immunized with FVB splenocytes 4‐6 wk prior to chimerism induction. For chimerism induction, bortezomib and cyclophosphamide was given on day ‐4 with respect to the date of bone marrow transplantation (BMT). T cell–depleting (TCD) antibodies were administered i.p. on day ‐2, 2, 6, 11, and 16. Some recipients that had been sensitized to FVB splenocytes were treated with EndoS‐imlifidase iv on day ‐6 and a repeated dose on day 0 at 4 h before BMT. Six Gy total body irradiation was given at 4 h prior to BMT on day 0. FVB bone marrow cells (80 × 10⁶) were given on day 0. B, Shown are the proportions of donor cells in lymphocyte gate in peripheral blood over time. C, Shown are percentages of different lineages of donor cells in lymphocyte gate in peripheral blood from naïve NOD chimeras (n = 4, on the left, mean ± SEM) and primed NOD chimeras (n = 2, on the right). Data were pooled from six independent experiments