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Cysteine dependent homodimer formation in ToxR. V. cholerae ΔtoxRS strains carrying toxR derivatives with or without its operon partner toxS on pFLAG‐MACTM were grown in LB. Samples were taken after 2 hr induction with 0.05 mM IPTG in the mid‐log phase and analyzed by immunoblotting using anti‐FLAG antibodies. Immunoblots were carried out under standard non‐reducing Laemmli buffer conditions
