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. 2020 May 28;50(9):1386–1399. doi: 10.1002/eji.201948455

Figure 7.

Figure 7

PI3Kδ reduces differentiation of human Meso‐CAR T cells while preserving cytolytic capacity. Healthy donor T cells were stimulated with anti‐CD3/CD28 beads and transduced with a mesothelin‐specific 4‐1BBζ CAR in the presence or absence of PI3K inhibitors. (A) Expansion of vehicle and inhibitor‐treated CAR T cell cultures over 2 weeks. (B) Representative flow cytometry plots of CD4+ CAR+ (top) and CD8+ CAR+ (bottom) T cell memory profiles on Day 7 of expansion. Expression of costimulatory and inhibitory receptors of CD4+ CAR+ (C) and CD8+ CAR+ (D) T cells on Day 7 of expansion. (E) Representative flow cytometry plots and quantification of K562‐meso cell death with total T cells in inhibitor‐free media on Day 15. Cell growth analyzed by one‐way ANOVA with Tukey's multiple comparisons at each time point. Surface expression and cytotoxicity data analyzed by one‐way ANOVA with Tukey's multiple comparisons, n = 6 healthy donors/group from two independent experiments. All bars represent the mean ± the SD with statistical significance as p < 0.05*, p < 0.01**, p < 0.001***, and p < 0.0001****.