Figure 5.

Methylation states of the promoter region of proviral petunia vein clearing caulimovirus (PVCV) in petals. Genomic DNAs were prepared from pigmented (2m P) and white (2m W) regions of petals of 2‐month‐old plants and pigmented (3m P), white (3m W), and blotched (3m B) regions of petals of 3‐month‐old plants, and treated using a bisulfite reagent. The promoter region of proviral PVCV was amplified by PCR with provirus‐specific primers (Table S3) and sequenced. The ratios of methylated cytosines in CG (red), CHG (blue), and CHH (green) sites were calculated from sequencing data of more than 10 PCR clones. Detailed presentation of methylated cytosines that are indicated as coloured circles. Filled colour circles, red (CG), blue (CHG), and green (CHH), indicate methylated cytosines, and open circles indicate unmethylated cytosines.