Table 1.
The sequences of amino acid elongators used in this study and their acylation yields and translation efficiencies. (N.D. indicates the product was detected but the yield was not determined)
|
Entry |
Foldamer sequence |
Acylation yield [%] |
Translation yield [%][a] |
|
|---|---|---|---|---|
|
|
|
|
T1 |
T2 |
|
1 |
Amf(‐Gly‐QDap‐Ac) |
20 |
92 |
68 |
|
2 |
Amf(‐Gly‐QAsp‐Ac) |
55 |
82 |
62 |
|
3 |
Amf(‐Gly‐(QDap)2‐Ac) |
24 |
48 |
11 |
|
4 |
Amf(‐Gly‐(QAsp)2‐Ac) |
29 |
– |
N.D.[b] |
|
5 |
Amf(‐Gly‐QDapP‐Ac) |
24 |
35 |
10 |
|
6 |
Amf(‐Gly‐QAspP‐Ac) |
0 |
– |
– |
|
7 |
Amf(‐Gly‐QDapPQDap‐Ac) |
10 |
5 |
N.D.[b] |
|
8 |
Amf(‐Gly‐QAspPQAsp‐Ac) |
0 |
– |
– |
|
9 |
Amf(‐Gly‐(QDap)3‐Ac) |
0 |
– |
– |
[a] The values provided are based on the observed incorporation efficiency when compared with the wild type expression (absence of reprogramming). [b] Low yields and band overlap hampered their identification and subsequent quantification (Supporting Information, Figures S4 and S5).