Skip to main content
. 2020 Mar 26;147(7):1987–1999. doi: 10.1002/ijc.32965

Figure 3.

Figure 3

DUSP6 is required for maintaining an invasive phenotype and for promoting metastasis. (a) Representative bright‐phase images of migration and invasion in DUSP6‐knockdown Hec1 cells after 36 hr. Scale bar, 100 μm. (b) Bar graph showing quantification of the number of migrating (left) and invading (right) DUSP6‐knockdown Hec1 cells after 36 hr. (c) Representative bright‐phase images of migration and invasion in DUSP6‐knockdown HHUA cells after 48 hr. Scale bar, 100 μm. (d) Bar graph showing quantification of the number of migrating (left) and invading (right) DUSP6‐knockdown HHUA cells after 48 hr. Error bars indicate the standard deviation. Data are representative of at least three independent experiments. (e) Tumor volumes, assessed by the IVIS imaging system, in mice at 35 days after tail vein injection of control and DUSP6‐knockdown Hec1 cells. (f) Tumor volumes, quantitated by measuring luciferase activity (bioluminescence intensity), in mice at 35 days after tail vein injection. (g) IVIS images showing luciferase activity in the tumors of excised lungs. (h) Volumes of lung metastases quantitated by measuring luciferase activity (bioluminescence intensity). The volumes of tumors derived from injection of DUSP6‐knockdown cells were smaller than those derived from injection of control cells. (i) Representative hematoxylin‐ and eosin‐stained images of excised lungs. Scale bar, 100 μm. (j) Tumor volumes, assessed by the IVIS imaging system, in mice injected with intrasplenic control and DUSP6‐knockdown Hec1 cells. (k) Tumor volumes, quantitated by measuring luciferase activity (bioluminescence intensity), in mice after injection of intrasplenic control and DUSP6‐knockdown Hec1 cells. (l) IVIS image showing luciferase activity in a liver metastatic tumor. (m) The bar graph shows quantification of the luciferase activity in the metastatic tumor. (n) Representative hematoxylin‐ and eosin‐stained images of excised livers. Scale bar, 100 μm. Error bars indicate the standard error of the mean. *p < 0.05, **p < 0.01, ***p < 0.005.