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The expression and purification of the His‐HaADH5 fusion protein. (A) Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) for the protein products of the Transetta (pET32a‐HaADH5) strain with isopropyl β‐D‐1‐thiogalactopyranoside (IPTG) induction. (B) SDS‐PAGE tracking the His‐HaADH5 protein purification progress. (C) Western blotting of the purified fusion protein with anti‐His tag mouse monoclonal antibody.
