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. 2019 Sep 12;27(5):1053–1066. doi: 10.1111/1744-7917.12720

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© 2019 The Authors. Insect Science published by John Wiley & Sons Australia, Ltd on behalf of Institute of Zoology, Chinese Academy of Sciences

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Analysis of the interaction between HaADH5 and the HE1 fragment of the CYP6B6 promoter. (A) Interaction of the HE1 fragment and different amounts of HaADH5 protein. (B) Electrophoretic mobility shift assay (EMSA) competition analysis of HaADH5 and the HE1 fragment. Lane 1: negative control without protein; Lane 2: positive control with 2 μg protein; Lane 3: competition reaction with 2 μg protein and 200‐fold unlabeled HE1 fragment; Lane 4: non‐competition reaction with 2 μg protein and 200‐fold unrelated bHLH fragment. (C) The interaction between HaADH5 and the 2‐tridecanone (2‐TD) responsive region of the CYP6B6 promoter in yeast.