Table 1.

Primers used in this study

Primer	Sequence (5′ to 3′)	Purpose
5′‐outer	CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT	HaADH5 5′ RACE outer PCR
5′‐P1	TCATCCAACGCCACACAAGG
5′‐inner	CTAATACGACTCACTATAGGGC	HaADH5 5′ RACE inner PCR
5′‐P2	GCAGGTTGTAGGATAGGTGCCTTGGG
3′‐outer	TACCGTCGTTCCACTAGTGATTT	HaADH5 3′ RACE outer PCR
3′‐P1	CTATCATCGATCCCAACGACAAAA
3′‐inner	CGCGGATCCTCCACTAGTGATTTCACTATAGG	HaADH5 3′ RACE inner PCR
3′‐P2	TTGTCAGCCCAAAGCAGGT
HaADH5‐F	GAGGTACCATGGTGAAGGCACGGAAA, Kpn Ι	HaADH5 ORF amplification for ligation into pET32a
HaADH5‐R	GCGTCGACTTATAATTTAACAACAGCCT, Sal Ι
HaADH5‐yF	CCATATGATGGTGAAGGCACGGAAATA, Nde I	HaADH5 ORF amplification for ligation into pGADT7
HaADH5‐yR	CCTCGAGTTATAATTTAACAACAGCCTTTC, Xho I
β‐Actin‐QF	CACACCTTCTACAACGAGCTG	qPCR of target and reference genes
β‐Actin‐QR	GAGGATCTTCATGAGGTAGTCG
Tubulin‐QF	TCCAACTCACACACTCGCT
Tubulin‐QR	GGAAGCAGATGTCGTATAATG
HaADH5‐QF	GTCTCCGCGGCGCTCAAA
HaADH5‐QR	TCATCCAACGCCACACAAGG
CYP6B6‐QF	TTCAAACTTATACCATGTCCACAAT
CYP6B6‐QR	CCAATTGACGGAGCTCTAGAATCA

PCR, polymerase chain reaction; RACE, rapid amplification of complementary DNA ends; ORF, open reading frame.