Table 1.
Information of the primary antibodies used in this study
| Antibody | Specificity | Type | Item numbers | Dilution | Source |
|---|---|---|---|---|---|
| β‐Actin | β‐Actin | Monoclonal | #3700 | 1:10,000 WB | Cell Signaling |
| Bcl‐2 | Total Bcl‐2 | Polyclonal | #15071 S | 1:1,000 WB | Cell Signaling |
| Caspase 3 | Total caspase 3 | Polyclonal | #9662 | 1:1,000 WB | Cell Signaling |
| Cleaved caspase 3 | Cleaved caspase 3 | Polyclonal | #9664 | 1:1,000 WB | Cell Signaling |
| mTOR | Total mTOR | Polyclonal | #2983 | 1:1,000 WB | Cell Signaling |
| p‐mTOR | p‐mTOR at Ser2448 | Polyclonal | # 5536 | 1:1,000 WB | Cell Signaling |
| P62 | SQSTM1/p62 | Polyclonal | #23214 | 1:1,000 WB | Cell Signaling |
| LC3α/β | LC3A/B | Polyclonal | #4108 | 1:1,000 WB | Cell Signaling |
| LC3β | LC3B | Polyclonal | #2775 | 1:100 IF | Cell Signaling |
| GFAP | GFAP | Polyclonal | #80788 | 1:50 IF | Cell Signaling |
| LAMP1 | LAMP1 | Monoclonal | #9091 | 1:100 IF | Cell Signaling |
| Cyclin D1 | Cyclin D1 | Monoclonal | #55506 | 1:1,000 WB | Cell Signaling |
| FAK | Total FAK | Polyclonal | #71433 | 1:1,000 WB | Cell Signaling |
| p‐FAK | p‐FAK at Try397 | Polyclonal | #8556 | 1:1,000 WB | Cell Signaling |
| Src | Total Src | Polyclonal | # 2108 | 1:1,000 WB | Cell Signaling |
| p‐Src | p‐Src at Try416 | Polyclonal | PA5–97364 | 1:50 IF | Thermo Fisher |
| Akt | Total Akt | Monoclonal | #4691 | 1:1,000 WB | Cell Signaling |
| p‐Akt (Ser473) | p‐Akt at Ser473 | Polyclonal | #4060 | 1:1,000 WB | Cell Signaling |
| CD34 | CD34 | Polyclonal | ab81289 | 1:100 IF | Abcam |
| CD63 | CD63 | Polyclonal | ab59479 | 1:1,000 WB /1:100 IF | Abcam |
| VE‐Caherin | VE‐Caherin | Polyclonal | ab33168 | 1:100 IF | Abcam |
| HSP70 | HSP70 | Monoclonal | #4873 | 1:1,000 WB | Cell Signaling |
| TSG101 | TSG101 | Polyclonal | ab125011 | 1:1,000 WB /1:100 IHC | Santa Cruz |
Abbreviations: Bcl‐2, B‐cell lymphoma 2; FAK, focal adhesion kinase; GFAP, glial fibrillary acidic protein; IF, immunofluorescence; IHC, immunohistochemistry; LAMP1, lysosome‐associated membrane protein 1; LC3A/B, light chain 3α/β; mTOR, mammalian target of rapamycin; p, phosphorylated; WB, western blot analysis.