Figure 1.
Monocytes from AS patients show an increase in CD74‐p8 accumulation. (A–C) CD14+ monocytes were isolated from PBMCs from HC, RA, PsA, and AS by CD14 magnetic beads. (A, B) CD14+ monocytes were incubated with the vehicle control DMSO, 1 μM (Z‐LL)2 ketone, or 20 nM LHVS for 24 h. At least two HCs were included on each Western blot. The protein lysates were tested for CD74 using an antibody directed against the N‐terminus (clone: 2D1B3). The blot was stripped and reprobed for β‐actin as a loading control. Representative blots. (C) CD74‐p8 was normalized to CD74‐p33/p35 (CD74‐FL) following normalization to a HC. Data combined from 16 independent experiments with seven to eight samples per experiment. The median is represented in the dot plot. ***p < 0.001; unpaired t test.