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Representative spectra identified in AAV2 capsid for ubiquitination modification at residue K544 (n = 2 replicates) (A) and phosphorylation modification at S149 residue (n = 4 replicates) (B). Mass spectrometry data of trypsin digested peptides from AAV2 was acquired on QExactive and analyzed using PEAKS studio 8.0. Peptide identification and modification assignment was performed from tandem mass‐spectrometry of the selected precursors.
