Table 3.
Post‐translational modifications present on AAV2‐AAVrh10 serotypes after liquid chromatography and mass‐spectrometry (LC/MS) and PEAKS analysis. Ph‐Phosphorylation, Ub‐Ubiquitination, Hx‐HexNAcylation, Ac‐Acetylation, Ng‐N‐linked glycan core, Su‐ SUMO‐1 or SUMO‐2/3.
| Serotype | Site | Modification | Peptide sequence |
|---|---|---|---|
| AAV2 | S149a | Phosphorylation | RPVEHS PhPVEPDSSSGTGK |
| N253 | Hex(4) HexNAc(2) | TWALPTYN NgNHLYKQIS | |
| K258 | SUMOylation by SUMO‐2/3 | TWALPTYNNHLYK SuQIS | |
| N518 | HexNacylation | LNGRDSLVN HxPGPAMASHKDDEEK | |
| S537 | dHex Hex(3) HexNAc(6) | EKFFPQS NgGVLIFGK | |
| K544a | Ubiquitination | SGVLIFGK Ub | |
| N551 | HexNAcylation | KTN HxVDIEKVMITDEEEIR | |
| AAV3 | A2a | Acetylation | A AcADGYLPDWLEDNLSEGIR |
| Y6a | Phosphorylation | AADGY PhLPDWLEDNLSEGIR | |
| S149a | HexNAcylation | KKGAVDQS HxPQEPDSSSGVGK | |
| AAV4 | K479 | Ubiquitination | KNWLPGPSIK UbQQGFSK |
| AAV5 | K122a | Ubiquitination | KAVFQAKK UbR |
| N292a | N‐linked Glycan core | RLIN NgNYWGFRPRS | |
| N308a | HexNAcylation | KIFN HxIQVKE | |
| K312 | SUMOylation by SUMO‐2/3 | VKIFNIQVK Su | |
| T376a | HexNAcylation | RDNT HxENPTERS | |
| N428 | dHex Hex(3) HexNAc(5) | LAN NgPLVDQYLYR | |
| K451a | Ubiquitination | RFVSTNNTGGVQFNK UbN | |
| K639a | Ubiquitination | KHPPPMMLIK UbN | |
| S680a | Phosphorylation | KENS PhKRW | |
| AAV6 | A2a | Acetylation | MA AcADGYLPDWLEDNLSEGIRE |
| AAV7 | A2a | Acetylation | MA AcADGYLPDWLEDNLSEGIRE |
| K61a | SUMOylation by SUMO‐2/3 | KYLGPFNGLDK SuGEPVNAADAAALEHDKA | |
| S157 | HexNAcylation | SPDSS HxTGIGKKGQQPARK | |
| T252 | Phosphorylation | TWALPT PhYNNHLYK | |
| N254 | HexNAcylation | TWALPTYN HxNHLYK | |
| N460 | HexNAcylation | TQSNPGGTAGN HxR | |
| AAV8 | A2a | Acetylation | MA AcADGYLPDWLEDNLSEGIRE |
| Y6a | Phosphorylation | MAADGY PhLPDWLEDNLSEGIRE | |
| N521 | N‐linked Glycan core | NSLAN HxPGIAMATHK | |
| AAV9 | A2a | Acetylation | MA AcADGYLPDWLEDNLSEGIRE |
| Y52a | Phosphorylation | KY PhLGPGNGLDKG | |
| N57a | HexNAcylation | KYLGPGN HxGLDKG | |
| K84a | SUMOylation by SUMO‐1 | AYDQQLK SuA | |
| K105 | Ubiquitination | LK UbEDTSFGGNLGR | |
| K316 | SUMOylation by SUMO‐2/3 | RLNFK Su | |
| T450 | Phosphorylation | T PhINGSGQNQQTLK | |
| S454a | Phosphorylation | TINGS PhGQNQQTLK | |
| K557 | SUMOylation by SUMO‐1 | DNVDADK Su | |
| K650a | Ubiquitination | HPPPQILIK UbN | |
| T702a | dHex Hex(3) HexNAc(3) | RWNPEIQYT NgSNYYK | |
| AAVrh10 | A2a | Acetylation | A AcADGYLPDWLEDNLSEGIR |
| Y90 | Phosphorylation | AGDNPY PhLR | |
| S149a | Phosphorylation | TAPGKKRPVEPS PhPQR | |
| S157 | HexNAcylation | SPDSS HxTGIGK | |
| T252 | Phosphorylation | TWALPT PhYNNHLYK | |
| N306 | N‐linked glycan core | LINNN NgWGFRPK | |
| T332a | HexNAcylation | EVTQNEGT HxKT | |
| K333a | Ubiquitination | EVTQNEGTK UbT | |
| S449 | HexNAcylation | LMNPLIDQYLYYLS HxR | |
| K652 | Ubiquitination | HPPPQILIK Ub | |
| K709 | SUMOylation by SUMO‐2/3 | RWNPEIQYTSNYYK Su |
a Denotes modification site identified by spectra in multiple runs (>1 run).