Table 6.
Novel post‐translational modification sites detected in AAV serotypes by liquid chromatography‐mass spectrometry (LC/MS) analysis.
| Serotype | Site | Modification |
|---|---|---|
| AAV2 | S149 | Phosphorylation |
| N253 | Hex(4) HexNAc(2) | |
| N518 | HexNAcylation | |
| N551 | HexNAcylation | |
| AAV3 | A2 | Acetylation |
| Y6 | Phosphorylation | |
| S149 | HexNAcylation | |
| AAV5 | K122 | Ubiquitination |
| N292 | N‐lined Glycan core | |
| N308 | HexNAcylation | |
| K312 | SUMOylation by SUMO‐2/3 | |
| T376 | HexNAcylation | |
| N428 | dHex Hex(3) HexNAc(5) | |
| K451 | Ubiquitination | |
| K639 | Ubiquitination | |
| S680 | Phosphorylation | |
| AAV6 | A2 | Acetylation |
| AAV7 | K61 | SUMOylation by SUMO‐2/3 |
| S157 | HexNAcylation | |
| T252 | Phosphorylation | |
| N254 | HexNAcylation | |
| N460 | HexNAcylation | |
| AAV8 | A2 | Acetylation |
| Y6 | Phosphorylation | |
| N521 | N‐linked Glycan core | |
| AAV9 | Y52 | Phosphorylation |
| N57 | HexNAcylation | |
| K84 | SUMOylation by SUMO‐1 | |
| K105 | Ubiquitination | |
| K316 | SUMOylation by SUMO‐2/3 | |
| T450 | Phosphorylation | |
| S454 | Phosphorylation | |
| K557 | SUMOylation by SUMO‐1 | |
| K650 | Ubiquitination | |
| T702 | dHex Hex(3) HexNAc(3) | |
| AAVrh10 | Y90 | Phosphorylation |
| S149 | Phosphorylation | |
| S157 | HexNAcylation | |
| N306 | N‐linked Glycan core | |
| T332 | HexNAcylation | |
| S449 | HexNAcylation | |
| K652 | Ubiquitination | |
| K709 | SUMOylation by SUMO‐2/3 |