. 2020 Jun 23;103(5):1924–1936. doi: 10.1111/tpj.14813

Table 3.

Estimation of insertion lines required to mutate the Brachypodium distachyon genome

Percentage chance of mutating a particular gene (%)	Tnt1 inserts required (N) ^a	Insertion lines required (IL) ^a
50	56 771	8915
65	85 984	13 502
75	113 543	17 829
85	155 381	24 399
90	188 590	29 613
95	245 362	38 528
99	377 181	59 227

^{^a}

Given an average gene length of 3.37 kb, the percentage chance of mutating a particular gene would require (N) Tnt1 inserts with a 99.39% chance of insertion into a sequenced gene space and a 42.15% chance of insertion into the gene transcript region. The number of insertion lines (IL) required is based on an insertion rate of 6.37 insertions per line (IL = N/6.37).