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Ethidium bromide enhances the ligation of a 2‐methylimidazole activated dinucleotide to a 3′‐amino primer. Reactions contained 2 μm primer, 4 μm template, 200 mm HEPES, pH 8.0, 0.2–10 mm 2‐MeImp‐GA. Data points are reported as the mean±s.d., n≥3.
