Figure 6.

LINC02595 acts as a miR‐203b‐3p sponge. (a) The distribution of LINC02595 in RKO and HT29 cells were detected by qRT‐PCR. (b) FISH analysis of the subcellular location of LINC02595 in RKO and HT29 cells. (c) The HEK293T cell was cotransfected with wild‐type (WT) LINC02595 plasmid and miR‐3942‐3p, miR‐4715‐3p, miR‐203b‐3p mimics or NC, and the luciferase activities were measured. *p < .05. (d) Dual‐luciferase reporter assay was performed with WT‐LINC02595 and MT‐LINC02595 luciferase report vectors in HEK293T cell. The left panel was the predicted target site for miR‐203b‐3p in LINC02595. *p < .05. (e) The miR‐203b‐3p expression was detected in 116 paired CRC tissues compared with adjacent nontumor tissues. *p < .05. (f) The miR‐203b‐3p expression was detected in five CRC cell lines compared with normal colorectal epithelial tissues. *p < .05. (g) The LINC02595/miR‐203b‐3p expression was detected in miR‐mimic or miR‐inhibitor/LIN‐si or LIN‐pcDNA transfected CRC cells. *p < .05. (h) Correlation analysis between LINC02595 expression and miR‐203b‐3p expression in 116 paired tissues (r = −0.323; p < .05). CRC, colorectal cancer; FISH, fluorescence in situ hybridization; GAPDH, Glyceraldehyde 3‐phosphate dehydrogenase; miR, microRNA; NC, negative control; qRT‐PCR, quantitative real‐time polymerase chain reaction