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. 2020 Jul 7;59(36):15560–15564. doi: 10.1002/anie.202005727

Figure 2.

Figure 2

Exo‐ and endoglycosidase digestions of DC‐SIGN CRD. a) Mass spectrum of CRD without enzymatic treatment indicates extensive sialylation of at least six glycoforms in each charge state. b) Sialidase digestion results in five peaks with different hexose and N‐acetylhexosamine compositions. c) Sequential digestion with sialidase and β1‐4 galactosidase reveals the presence of both Core 1 and Core 2 O‐glycan structures, (inset in (b)). d) Replacing β1‐4 galactosidase with O‐glycanase, which is specific for Core 1 O‐glycans, results in the formation of the non‐glycosylated CRD (apo peak) and the identification of β1‐4 galactose extensions on Core 2 O‐glycans. e) Schematic of CRD glycan structures generated from exo‐ and endoglycosidase digestions. f) DC‐SIGN CRD glycan macroheterogeneity and microheterogeneity identified from exoglycosidase sequencing.