Table 5.
Gene | Forward primer 5′ → 3′ | Reverse primer 5′ → 3′ | Accession # |
---|---|---|---|
YWHAG | TCCATCACTGAGGAAAACTGCTAA | TTTTTCCAACTCCGTGTTTCTCTA | XM_005661962.3 |
PKM2 | ATGCAGTCTTGGATGGAGCTGACT | ATTGCAAATGGTAGATGGCGGCCT | AJ557236.1 |
SLC2A1 | TCCACACCCACTTTGTCACACTGA | AGCCTCAACTCCCACATCACTGAA | XM_021096908.1 |
SLC2A3 | CCCTCAGCTGCATTCTATTT | GTCTCAGGGACTTTGAAGAAG | XM_021092392.1 |
PGK1 | CGCTTTCTGCATCTCCACTTGGCA | GCTGTGCAATGGTTCAAGGGTTCCT | NM_001099932.2 |
PDK1 | ACCAGGACAGCCAATACAAGTGGT | ACGTGGACTTGAATAGGCGGGTAA | NM_001159608.1 |
TALDO1 | TGAAGCGGCAGAGGATGGAGAGC | TCGTCGATGGCGTTGAAGTCGC | NM_001244935.1 |
EPAS1 | AAGCAAAGACATGTCCACCGAGCG | GTGGCTGACTTGAGGTTGACGGTG | NM_001097420.1 |
HK1 | TCTTGATCGACTTCACCAAGAGGG | TCGCTCTCGATCTGCGAGAGATACTT | NM_001243184.1 |
HK2 | GAATTTGATGCGGCCGTGGATGAA | CCAGGTACATGCCGCTGATCATTT | NM_001122987.1 |
ENO1 | TCGGAGTTCTACAGGTCGGGCAAG | TGGTCCGGTGAGATGTACCTGCTG | XM_021095280.1 |
PGAM1 | CAGTGCTGGATGCCATTGACCAAA | GCTTGGCAGCAGTTTCTGCCTTAT | XM_003483535.4 |
LDHA | TTCAGCCCGGTTCCGTTACCTAAT | TTCTTCAGGGAGACACCAGCAACA | NM_001172363.2 |
LDHB | TAAGCATGGGCTTTGACTCTGGGA | ACTCCCGGCTTCTAGGTTGTAGTA | NM_001113287.1 |
VEGFA | CAAACCTCACCAAGGCCAGCACAT | CGAGCAAGGCCCACAGGGATTTTC | NM_214084.1 |
GPI | CCAGGAGACCATCACAAATG | TAGACAGGGCGACAAAGT | NM_214330.1 |
ALDOC | TCTTCCATGAGACCCTCTAC | TACACCCTTGTCCACCTT | NM_001243928.1 |
BNIP3 | GGATTACATGGAGAGGAGGA | GTGCTTGAAGAGGAGGAAC | XM_003359404.4 |
BCL2 | ACTGAATGCCCTCCGGTACC | ATCCCCATGGCTGCAGTGAA | XM_003130557.2 |
ACTB | TCTGGCACCACACCTTCT | TGATCTGGGTCATCTTCTCAC | DQ178122.1 |
POU5F1 | TTTGGGAAGGTGTTCAGCCAAACG | TCGGTTCTCGATACTTGTCCGCTT | NM_001113060.1 |
Abbreviation: RT‐PCR, real‐time polymerase chain reaction.