Figure 1.

Maternal dietary intake of linseed oil ameliorates infant CHS. Pups were nursed for 2 weeks by dams maintained on diets containing 4% soybean oil (Soy) or linseed oil (Lin), after which infant mice were sensitized to DNFB. Five days after sensitization, infants were challenged with DNFB, and (A) ear swelling was calculated as the difference between ear thickness before and at 48 h after DNFB challenge. Horizontal lines indicate median values. P values were obtained by using the Mann‐Whitney U test (***P < .001). The graph shows data from individual infant mice (n = 48 [Soy] or 60 [Lin]); data were pooled from four representative independent experiments with reproducible results. (B) Frozen ear sections were stained with hematoxylin and eosin and analyzed microscopically at 48 h after DNFB challenge. Data are representative of three independent experiments with reproducible results. Bar, 100 μm. (C) Representative plots and numbers of IFN‐γ^high–producing CD8α⁺ T cells in infant skin at 48 h after DNFB challenge, as determined by using flow cytometry. The cell number shown is for the left (ie, challenged) ear of each pup. Horizontal lines indicate median values. P values were obtained by using the Mann‐Whitney U test (*P = .0173). Data from the left ear of individual mice are shown and are representative of three independent experiments with reproducible results