Figure 5.

Maternal intake of linseed oil leads to the accumulation of 14‐lipoxygenation products of ω3 DPA in mouse pups due to the unique enzymatic environment in the mammary gland. (A) Representative chromatographs of the precursor ion at m/z 345 were obtained for the 14‐lipoxygenation products of DPA and of infant sera after maternal intake of linseed oil and soybean oil. Representative MS spectra (retention time [RT] 25.06‐25.08 min) from three independent experiments are shown. (B) The peak area of the precursor ion at m/z 345 was obtained for each 14‐lipoxygenation product of DPA in the sera of infants born to linseed oil– compared with soybean oil–fed dams. Horizontal lines indicate median values. P values were obtained by using the two‐tailed unpaired t test (*P < .05). (C) The mRNA expression levels of lipid metabolic genes (Elovl2, Elovl5, and Alox12) in the abdominal mammary glands (MG) and livers of 12‐week‐old dams, which had lactated for 2 weeks. Horizontal lines indicate median values. P values were obtained by using the Mann‐Whitney U test (*P < .05; ns, not significant). Data are representative of two independent experiments with reproducible results