Figure 7.

Compounds (±)‐4 and 4 significantly reduced the cytokine production and transcription factor expression in Th1 and Th17 cells but did not exhibit immunomodulatory capacities. A)–C) Human CD4⁺ T cells were sorted from peripheral blood and activated with anti‐CD3 and anti‐CD28 for 48 h (activated CD4). Subsequently, cells were stimulated with compounds (±)‐4, 4 and ent‐4 (1 μM each) for an additional 48 h. Control cells received an equal amount of PBS. A) Representative dot‐plots and percentages of cells expressing B) the Th1 markers IFN‐γ and T‐bet, the Th17 markers IL‐17 and ROR‐c from n=4 healthy human donors or C) the Treg markers Foxp3 and Helios are shown. Cytokine and transcription factor staining were performed after cell permeabilization. Data are presented as mean±SEM; * p <0.05. D)–F) Mouse CD4⁺ T cells were sorted from spleen and lymph node tissue of wild‐type mice, activated with anti‐CD3 and anti‐CD28 for 48 h (activated CD4), and stimulated with compounds (±)‐4, 4 and ent‐4 (1 μM each) for an additional 48 h. D) Representative dot‐plots and percentages of cells expressing E) the Th1 markers IFN‐γ and T‐bet, the Th17 markers IL‐17 and ROR‐γt from n=4 individual mice or F) the Treg markers Foxp3 and Helios are shown. Cytokine and transcription factor staining were performed after cell permeabilization. Data are presented as mean±SEM; * p <0.05.