FIGURE 9.

Assessment of fibrosis‐associated genes expressions in NRVFs after GATA4 and GATA4‐S105A adenovirus‐mediated overexpression for 48 hrs. The experiments are grouped into 3 sets according to the adenovirus concentration: 2 MOI (+; ++) and 4 MOI (+++). GATA4 overexpression (A) and the mRNA levels of fibrotic genes FN1, Col1a1, Col3a1, TGFβ1, MMP2, MMP9, TIMP1, FGF2 and PEX1 (B‐J) were quantified by RT‐qPCR. The expression values were normalized to GAPDH and are expressed as relative to LacZ control groups (n = 3‐4/group). The results are represented as average + SD. * P ≤ .05, ** P ≤ .01, *** P ≤ .005 vs LacZ. # P ≤ .05, ## P ≤ .01, ### P ≤ .005 vs GATA4