Figure 11.
PLA reveals low level colocalization of SK2‐Cav1.2α1c complexes with RyR2 in Sham and TAB rat VMs
A, PLA of SK2 and Cav1.2α1c, and colocalization with RyR2. Left: each red fluorescent dot represents a site of interaction of proteins that are within 40 nm proximity. Centre: cells were probed with anti‐RyR2 antibody. Right: merged image showing colocalization of SK2 protein pairs with RyR2. B, PLA and RyR2 secondary antibodies only, as a technical control. C, quantification of the number of PLA puncta per μm2 in Sham and TAB VMs. No significant differences were observed between groups, Student's t test, Sham n = 29, TAB n = 28, N = 4 per group. D, Manders coefficients M1 and M2 reveals a low level of colocalization of SK2‐Cav1.2α1c with RyR2. No significant differences were observed between groups Student's t test: Sham, n = 31; TAB, n = 26; N = 4 per group.