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. 2019 Mar 20;598(14):2847–2873. doi: 10.1113/JP277618

Figure 19.

Figure 19

N‐terminus S136 does not confer PKA‐dependent changes in SK2 function

A, representative traces of [Ca2+]i transients and I SK at baseline and after incubation with ISO (100 nmol L–1) in VMs expressing the rSK2‐S136D mutant and dnPLB. B, pooled mean ± SD I–V and peak [Ca2+]/V relationships for (A). n = 6–8, N = 5–6 per group. I SK: * P = 0.02 (−35 mV), P = 0.001 (−25 mV), P = 5.8 × 10−4 (−15 mV), P = 0.005 (−5 mV), P = 0.02 (5 mV), P = 0.02 (15 mV), P = 0.01 (25 mV), P = 0.004 (35 mV), P = 0.002 (45 mV), P = 0.001 (55 mV) vs. baseline. ** P = 0.01 (−25 mV), P = 0.003 (−15 mV), P = 0.006 (−5 mV), P = 0.01 (5 mV), P = 0.01 (15 mV), P = 0.007 (25 mV), P = 0.002 (35 mV), P = 0.001 (45 mV), P = 0.001 (55 mV) vs. dnPLB. [Ca2+]: * P = 0.01 (−15 mV), P = 0.01 (−5 mV), P = 0.001 (5 mV), P = 5.1 × 10−4 (15 mV), P = 2.5 × 10−4 (25 mV), P = 1.7 × 10−4 (35 mV), P = 7.4 × 10−5 (45 mV), P = 1.5 × 10−4 (55 mV) vs. baseline. ** P = 0.049 (15 mV), P = 0.04 (25 mV), P = 0.02 (35 mV), P = 0.004 (45 mV), P = 0.002 (55 mV) vs. dnPLB, one‐way ANOVA with a Bonferroni post hoc test. C, representative western blots from VMs expressing rSK2 WT or rSK2‐S136D.