FIGURE 4.

NF‐κB is activated and participates in the AKIP1 mediated induction of the EMT. A, The cytoplasmic and nuclear proteins in Lv‐AKIP1‐HeLa, Lv‐AKIP1‐CaSki, and control LEV cells were extracted. Levels of indicated proteins were measured using western blot. B, Measurement of protein signal intensities using ImageJ software. C, AKIP1‐HeLa and Lv‐AKIP1‐CaSki cells were transfected with siRNA‐negative control (siNC) or p65 siRNA transiently for 72 hours to knock down p65. The expression levels of AKIP1, p65, E‐cadherin, and fibronectin proteins were detected using western blot analysis. D, Measurement of protein signal intensities was done using ImageJ software. E, Knockdown of p65 using siRNA in Hela and CaSki cells was confirmed using RT‐PCR. Mean ± SD is used for the description of data from three independent measurements. ** indicates P < .01, as compared to the control