FIGURE 6.

AKIP1 Suppresses PTEN during the EMT. A, HeLa and CaSki cervical cancer cells were transfected with a control siRNA (siNC) or AKIP1 siRNA. The cell lysates were collected and the protein expression of PTEN, AKIP1, p‐Akt, total Akt, and β‐Actin was detected using western blot. B, Stable AKIP1‐expressing cells were transfected with EV, wild‐type PTEN, or G129E‐mutant PTEN plasmids. At 48 hours after transfection, cell lysates were harvested after cell fractionation. Levels of indicated proteins were determined using western blot analysis