Table 1.
Apparent K M and app. k cat values for hydrolysis and transacylation by PnC_KS‐AT.
|
Substrate |
K M [μM] |
k cat [min−1] |
k cat/K M |
|---|---|---|---|
|
Hydrolysis |
|
|
|
|
2 |
53±41 |
0.6±0.15 |
0.01 |
|
3a |
73±33 |
1.4±0.3 |
0.02 |
|
3b |
6±1.8 |
0.9±0.06 |
0.15 |
|
3c |
6.2±1.4 |
0.7±0.04 |
0.11 |
|
3d |
20±6.7 |
0.8±0.08 |
0.04 |
|
3e |
19±15.5 |
0.4±0.08 |
0.02 |
|
3f |
–[a] |
– |
– |
|
Transacylation | |||
|
2 |
n.d[b] |
0.04±0.002 |
– |
|
3a |
n.d |
n.d |
– |
|
3b |
n.d |
4±0.1 |
– |
|
3c |
n.d |
0.6±0.03 |
– |
|
3d |
n.d |
0.3±0.07 |
– |
|
3e |
n.d |
0.05±0.001 |
– |
|
3f |
n.d |
– |
– |
[a] Not detected. [b] not determined.