Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 23;72(1):213–229. doi: 10.1002/hep.31002

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 The Authors. Hepatology published by Wiley Periodicals, Inc., on behalf of American Association for the Study of Liver Diseases.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

PMC Copyright notice

MyoVb deficiency does not disrupt canalicular protein localization. (A,B) Immunofluorescent staining of ABCC2 and radixin reveals their canalicular localization in both wild‐type and Myo5b KO mouse liver sections. HNF4α costaining marks hepatocytes. (C) KO of MYO5B in HepG2^KO cells (treated with MYO5B‐targeting pLentiCRISPR V2) was confirmed by western blot (compared with parental line, HepG2^PAR). (D) In HepG2 cells, localization of ABCC2 and F‐actin is unaffected by MYO5B KO (HepG2^KO) compared with HepG2^PAR control. Yellow arrowheads indicate BCs. (E) Western blot for myoVb in HUES9^KO cells confirmed MYO5B KO (compared with parental line, HUES9^Par). (F) HiHeps generated from HUES9^KO cells exhibit BC formation comparable with HUES9^Par‐derived hiHeps, with exclusive canalicular labeling of ABCC2. Scale bars: 10 μm.