Figure 2.

Immune responses of spleen cells upon immunization and therapeutic vaccination with the N‐terminal variable epitope library (VEL‐NT). (a, b) Mouse splenocyte pools (n = 3, each point represents cells from three independent experiments) were generated 15 days after vaccination with VEL‐NT and wild‐type (WT) ‐NT and analyzed by multiparametric flow cytometry. (b) CD8⁺ CD107a⁺ IFN‐γ ⁺ and CD4⁺ CD107a⁺ IFN‐γ ⁺ cell subsets were determined within proliferating cells. Spleen cells were also obtained from non‐tumor‐bearing mice, immunized with the same immunogens. Cell proliferation data (%) are presented as mean ± 95 CI and were analyzed with two‐way analysis of variance for repeated measurements and Sidak post‐hoc test for multiple comparisons. *P < 0·033, **P < 0·02, ***P < 0·001.