Table 2.
Panel of antigens used in cell proliferation assays
| WT‐NT | M | G | A | P | A | L | P | Q | I | W | Q | L | Y | L | K | N | Y | R | I | A | T | F | K | N | W | P | F | L | E | D | C | A | C | T | P | E | R | M | A | E |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| VEL‐NT | M | G | A | P | X | L | P | Q | I | W | Q | L | Y | L | X | N | Y | X | I | A | X | F | K | N | W | P | F | L | X | D | X | A | X | T | P | X | R | M | A | E |
| VEL‐NT | ||||||||||||||||||||||||||||||||||||||||
| derived antigen | ||||||||||||||||||||||||||||||||||||||||
| 1 | – | – | – | – | V | – | – | – | – | – | – | – | – | – | N | – | – | M | – | – | Q | – | – | – | – | – | – | – | I | – | N | – | R | – | – | L | – | – | – | – |
| 2 | – | – | – | – | R | – | – | – | – | – | – | – | – | – | T | – | – | P | – | – | R | – | – | – | – | – | – | – | A | – | S | – | M | – | – | S | – | – | – | – |
| 3 | – | – | – | – | Q | – | – | – | – | – | – | – | – | – | Q | – | – | A | – | – | Q | – | – | – | – | – | – | – | T | – | R | – | P | – | – | L | – | – | – | – |
| 4 | – | – | – | – | L | – | – | – | – | – | – | – | – | – | T | – | – | P | – | – | H | – | – | – | – | – | – | – | T | – | R | – | A | – | – | Q | – | – | – | – |
| 5 | – | – | – | – | I | – | – | – | – | – | – | – | – | – | G | – | – | A | – | – | A | – | – | – | – | – | – | – | Y | – | P | – | D | – | – | P | – | – | – | – |
| 6 | – | – | – | – | R | – | – | – | – | – | – | – | – | – | R | – | – | P | – | – | T | – | – | – | – | – | – | – | K | – | S | – | P | – | – | A | – | – | – | – |
| 7 | – | – | – | – | E | – | – | – | – | – | – | – | – | – | S | – | – | S | – | – | L | – | – | – | – | – | – | – | Y | – | P | – | G | – | – | L | – | – | – | – |
| 8 | – | – | – | – | S | – | – | – | – | – | – | – | – | – | Q | – | – | H | – | – | Y | – | – | – | – | – | – | – | T | – | P | – | I | – | – | V | – | – | – | – |
| 9 | – | – | – | – | M | – | – | – | – | – | – | – | – | – | V | – | – | L | – | – | K | – | – | – | – | – | – | – | D | – | H | – | F | – | – | T | – | – | – | – |
| WT‐CT | Q | M | E | E | L | T | V | S | E | F | L | K | L | D | R | Q | R | A | K | N | K | I | A | K | E | T | N | N | K | Q | K | E | F | E | E | T | A | K | T | T | R | Q | S | I | E | Q | L | A | A |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| VEL‐CT | Q | M | E | X | L | T | X | S | X | F | L | K | L | X | R | Q | X | A | K | N | K | X | A | K | E | T | N | N | K | Q | K | X | F | E | E | T | A | X | T | T | R | Q | S | I | E | Q | L | A | A |
| VEL‐CT | |||||||||||||||||||||||||||||||||||||||||||||||||
| derived antigen | |||||||||||||||||||||||||||||||||||||||||||||||||
| 1 | – | – | – | Q | – | – | N | – | P | – | – | – | – | P | – | – | P | – | – | – | – | F | – | – | – | – | – | – | – | – | – | A | – | – | – | – | – | G | – | – | – | – | – | – | – | – | – | – | – |
| 2 | – | – | – | R | – | – | H | – | I | – | – | – | – | N | – | – | E | – | – | – | – | Q | – | – | – | – | – | – | – | – | – | G | – | – | – | – | – | W | – | – | – | – | – | – | – | – | – | – | – |
| 3 | – | – | – | Q | – | – | E | – | I | – | – | – | – | P | – | – | T | – | – | – | – | A | – | – | – | – | – | – | – | – | – | A | – | – | – | – | – | R | – | – | – | – | – | – | – | – | – | – | – |
| 4 | – | – | – | I | – | – | R | – | K | – | – | – | – | V | – | – | T | – | – | – | – | H | – | – | – | – | – | – | – | – | – | Q | – | – | – | – | – | G | – | – | – | – | – | – | – | – | – | – | – |
| 5 | – | – | – | Q | – | – | N | – | P | – | – | – | – | P | – | – | P | – | – | – | – | F | – | – | – | – | – | – | – | – | – | A | – | – | – | – | – | G | – | – | – | – | – | – | – | – | – | – | – |
| 6 | – | – | – | Y | – | – | S | – | W | – | – | – | – | H | – | – | S | – | – | – | – | R | – | – | – | – | – | – | – | – | – | P | – | – | – | – | – | G | – | – | – | – | – | – | – | – | – | – | – |
| 7 | – | – | – | A | – | – | R | – | T | – | – | – | – | T | – | – | S | – | – | – | – | V | – | – | – | – | – | – | – | – | – | L | – | – | – | – | – | R | – | – | – | – | – | – | – | – | – | – | – |
| 8 | – | – | – | R | – | – | R | – | V | – | – | – | – | C | – | – | R | – | – | – | – | A | – | – | – | – | – | – | – | – | – | V | – | – | – | – | – | C | – | – | – | – | – | – | – | – | – | – | – |
| 9 | – | – | – | R | – | – | K | – | S | – | – | – | – | K | – | – | S | – | – | – | – | D | – | – | – | – | – | – | – | – | – | K | – | – | – | – | – | L | – | – | – | – | – | – | – | – | – | – | – |
X = Any of 20 amino acids. Amino acid sequences of antigen variants isolated from variable epitope libraries (VELs) at random, as well as wild‐type (WT) antigens, were deduced after DNA sequencing of individual phage clones.