Figure 4.

Mice under tear hyperosmolarity (THO) for 5 days do not develop clinical signs of dry eye disease (DED). Mice were exposed to isoosmolar (0·3 Osm/l, blue symbols) or hyperosmolar (3 Osm/l, red symbols) treatment for 5 days. (a) Tear production levels as assessed daily. (b) Corneal fluorescein staining score (National Eye Institute scale, left panel) and representative photos taken on day 5 (right). (c) Corneal irregularity score (left) and representative photos from day 5 (right). Pooled data (mean ± SD) from three independent experiments, 4–6 mice/group. ns indicates no statistically significant difference for treatment between groups by two‐factor anova (treatment and time).