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Ubiquitin‐specific protease 44 (USP44) overexpression inhibited the activation of the wingless/integration (Wnt)/β‐catenin pathway. Lentivirus oeUSP44 was transduced into HT29 and HCT116 cell lines, and cells were then cultured with or without 20 mmol/l LiCl. Forty‐eight hours later, the protein levels of β‐catenin, Axin1, c‐myc, and cyclin D1 were measured by western blot analysis
