Figure 6.

USP44 stabilized Axin1 via deubiquitination. (a) Lentivirus oeUSP44 was transduced into HT29 and HCT116 cell lines. Forty‐eight hours later, the mRNA level of Axin1 was measured by RT‐qPCR. (b) The interaction of USP44 and Axin1 in HT29 and HCT116 cell lines was detected by co‐immunoprecipitation analysis. (c) Lentivirus oeUSP44 was transduced into HT29 and HCT116 cell lines. Forty‐eight hours later, the level of Axin1 ubiquitination was detected by ubiquitination assay. IgG, immunoglobulin G; IP, immunoprecipitation; mRNA, messenger RNA; RT‐qPCR, real‐time reverse transcription quantitative polymerase chain reaction; USP44, ubiquitin‐specific protease 44