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. 2020 Jun 22;59(35):14950–14956. doi: 10.1002/anie.202004963

Figure 4.

Figure 4

a) Enzymatic cascade reaction for in vitro THIQ carboxymethylation. CM† can be omitted from the reaction since the rearrangement of chorismate to prephenate can proceed spontaneously. b) HPLC traces of cascade reactions. [I] mixed standards; [II] assay of WT CNMT and CmoA without optimisation; [III] assay of CNMT Y81R and CmoA without optimisation; [IV] assay of CNMT Y81R and CmoA following optimisation as shown in Figure S10 E. HPLC peaks: grey=starting material 4; red=carboxymethylation product 4 a; blue=methylation side product 4 b.