FIGURE 6.

BGJ398 impairs FGF9‐induced growth by shifting cells to G0/G1 phase of cell cycle and/or to apoptosis. Twenty‐four h after seeding, hepatoma/hepatocarcinoma cells were treated with DMSO (Co) or BGJ398; 2 h later cells were stimulated with 10 ng FGF9/ml medium. Fourty‐eight h later, FACS determined the percentage of cells in the cell cycle phases (A) or undergoing apoptosis (B). (C), Cells were counted at regular intervals after start of FGF9 treatment (time point 0). (A‐C), Data are expressed as means ± SEM of 3 independent experiments. Statistics by unpaired t‐test: any treatment without FGF9 vs any treatment + FGF9: a, P < .05; b, P < .01; any treatment without BGJ vs any treatment + BGJ: c, P < .05; d, P < .01. Statistics by One‐Sample t‐test for any treatment vs control: e, P < .05; f, P < .01