Figure 1.

Mono‐ and bidentate SAv binding to Bt‐modified sites on a DNA origami during continuous HS‐AFM scanning. a) Overview HS‐AFM image (1×1 μm²) recorded at the beginning of the experiment. Inset: A schematic of the Bt modifications on the DNA origami, consisting of a single Bt in the center of each trapezoid and a Bt dimer in one edge. The occupancy of b) a monodentate and c) a bidentate binding site on the DNA origami indicated in (a) is determined from each AFM image and monitored continuously over a time course of 10 min. d) Selected zooms of the tracked DNA origami. The data in (b) and (c) were determined for the two binding sites located on the upper trapezoid. Bound SAv molecules at these sites are indicated by white arrows. The HS‐AFM images (1×1 μm²) were recorded at a free amplitude of 3.3 nm, SR=0.7, LR=50 Hz, and a resolution of 512×512 px².