Table 2.

Influence of stilbenoid lead 3 and 6 f, 7 a–f, 11, 12 a–d, and 20 d on the deacetylase activity of human sirtuin isotypes Sirt1–3 determined in the fluorescence‐based ZMAL activity assay.

	Sirt1 inhibition[a]	Sirt2 inhibition[a]	Sirt3 inhibition[a]
3	27 % @ 50 μm	24.6±2.8 μm [b]	41.7±2.0 μm [b]
6 f	n.i.	n.i.	n.i.
7 a	14 % @ 50 μm	7.9±0.6 μm [b]	9.5±0.9 μm [b]
7 b	19 % @ 50 μm	10.8±0.6 μm [b]	7.9±0.5 μm [b]
7 c	n.i.	55 % @ 10 μm	64 % @ 10 μm
7 d	10 % @ 10 μm	62 % 10 μm	39 % @ 50 μm
7 e	17 % @ 10 μm	54 % @ 10 μm	66 % @ 10 μm
7 f	47 % @ 100 μm	5.8±0.7 μm [b]	9.4±0.7 μm [b]
11	n.i.	n.i.	n.i.
12 a	33 % @ 100 μm	45 % @ 100 μm	45 % @ 100 μm
12 b	n.i.	16 % @ 10 μm	11 % @ 10 μm
12 c	n.i.	17 % @ 10 μm	11 % @ 10 μm
12 d	n.i.	43 % @ 100 μm	43 % @ 100 μm
20 d	n.i.	49 % @ 10 μm	33 % @ 10 μm

[a] Percent inhibition relative to controls at the indicated concentration, [b] IC₅₀ values (μm) with statistical limits; values are mean±SD of duplicate experiments, n.i.: no inhibition detected (<30 % @ 100 μm).