Figure 4.

CEACAM1 does not mediate TIM‐3 signaling. (A) Schematic of WT and mutated TIM‐3 molecules (left) and flow cytometric analysis of TPR reporter cells expressing the indicated TIM‐3 molecules (gray histograms) and control TPR (open histogram). (B) The indicated TPR were stimulated with control TCS or TCS‐expressing CEACAM1. eGFP, eCFP, and mCherry expression was measured via flow cytometry. Results are shown for five independent experiments performed in triplicates. Reporter activation is shown as fold induction (gMFI of TCS‐stimulated cells/gMFI of unstimulated cells). For statistical evaluation, unpaired t‐tests were performed (ns, p > 0.05).