Figure 2.

MCF‐7‐Tam‐R cells are resistant to 4‐OH‐tamoxifen treatment and show enhanced motility and invasive behaviors and express higher SOX11 and slug. (a) Morphology of MCF‐7 and MCF‐7‐Tam‐R cells. (b) Cell viability analysis of MCF‐7 and MCF‐7‐Tam‐R cells after treatment with 4‐OH‐tamoxifen. (c) The cell invasion and migration capacity were examined using a Transwell assay in MCF‐7 and MCF‐7‐Tam‐R cells. (d) Immunofluorescence of MCF‐7‐Tam‐R cells and their parental cells stained with anti‐E‐ca (red signal) antibody, DAPI (blue signal). (e) Relative protein levels of SOX11, slug, ESR1, E‐ca, and vimentin were detected by western blot analysis in MCF‐7 and MCF‐7‐Tam‐R cells. (f) The mRNA levels of SOX11 and slug in MCF‐7 and MCF‐7‐Tam‐R cells were determined by real‐time RT‐PCR. DAPI, 4′,6‐diamidino‐2‐phenylindole; mRNA, messenger RNA; RT‐PCR, real‐time‐polymerase chain reaction