Figure 3.

SOX11 knockdown in MCF‐7‐Tam‐R cells decreases slug level and reversed the EMT process. (a and b). MCF‐7‐Tam‐R cells were transfected with two different sequences of SOX11 siRNAs. (c) Western blot analysis confirmed that SOX11 knockdown in MCF‐7‐Tam‐R cells could decrease the expression of slug and reverse the EMT process. (d) Cell viability was examined using the CCK‐8 assay after treatment with four different concentrations of 4‐OH‐tamoxifen for 72 hr in siNC transfected MCF‐7 cells, siNC transfected MCF‐7‐Tam‐R cells, and siSOX11 transfected MCF‐7‐Tam‐R cells. (e and f). Colony numbers were counted in the MCF‐7‐Tam‐R cells and siSOX11 transfected MCF‐7‐Tam‐R cells. CCK‐8, Cell Counting Kit‐8; EMT, epithelial–mesenchymal transition; mRNA, messenger RNA; siNC, siRNA negative control; siRNA, small interfering RNA