Figure 4.

SOX11 regulates the EMT process via slug. (a and b) MCF‐7‐Tam‐R cells were simultaneously transfected with siSOX11 and PCMV‐slug and the levels of SOX11, slug, and the EMT‐related proteins were analyzed by western blot analysis and real‐time RT‐PCR. (c and d) The cell invasion and migration capacity were examined using a Transwell assay in MCF‐7‐Tam‐R cells, cotransfected with siSOX11 and PCMV‐slug. (e) Cell viability analysis of MCF‐7‐Tam‐R cells, cotransfected with siSOX11 and PCMV‐slug after treatment with 4‐OH‐tamoxifen. EMT, epithelial–mesenchymal transition; mRNA, messenger RNA; RT‐PCR, real‐time‐polymerase chain reaction; siNC, siRNA negative control