Figure 5.

SOX11 regulates the expression of slug by binding to the slug promoter. (a) The human slug promoter sequence. (b) SOX11 binding on the slug promoter is shown using the ChIP assay. (c) The schematic diagram of constructs with a luciferase reporter plasmid. (d) Luciferase assays were performed in MCF‐7‐Tam‐R cells by cotransfection with siNC, siSOX11, PGL3 slug Wt, or PGL3 slug Mt and luciferase activity was normalized to the renilla minimal reporter. (e) Luciferase assays were performed in MCF‐7 cells by cotransfection with PCMV, PCMV‐SOX11, PGL3 slug Wt, or PGL3 slug Mt and luciferase activity was normalized to the renilla minimal reporter. (f and g) Luciferase assays were performed in MCF‐7‐Tam‐R cells by cotransfection with siSOX11, ESR1 promoter with or without PCMV‐slug, and luciferase activity was normalized to the renilla minimal reporter. (h) Schematic diagram showing enhanced SOX11‐mediated tamoxifen resistance by modulating slug expression. ChIP, chromatin immunoprecipitation; IgG, Immunoglobulin G; siNC, siRNA negative control